22 May 2022, 9:29 pm
Laboratory Animals
Laboratory Animals, Ahead of Print.
Consideration of The Three R’s (Replacement, Reduction, and Refinement) is essential when setting Humane Endpoints; however, a common interpretation assumes that Humane Endpoints are timepoints to perform euthanasia. This interpretation is not always consistent with the three Rs. There are many available intervention options that – when used to respond to pain, discomfort, or distress – facilitate application of the three Rs while achieving experimental goals. At our institution, the term ‘Humane Endpoints’ was replaced with ‘Humane Intervention Points’, to ensure responses beyond euthanasia are given priority by laboratory animal professionals and researchers. Identification of Humane Intervention Points in the research protocol provides us with a more accurate reflection of the measures used to alleviate pain and distress in animals used for research, testing, and teaching – an outcome easily envisioned elsewhere.Points d’intervention humains (PIH): Affinage de la terminologie d’évaluation finale pour intégrer des options d’intervention sans recours à l’euthanasie afin d'améliorer le bien-être des animaux et de préserver les résultats expérimentaux Résumé
12 May 2022, 10:55 pm
Laboratory Animals
Laboratory Animals, Ahead of Print.
Percutaneous catheterization is currently utilized for central venous access in adult and adolescent porcine models; however, neonatal models require a cut down to gain venous access. This approach requires general anesthesia and can result in systemic inflammation, which can confound studies investigating other inflammatory triggers. Here we present the first successful series of percutaneous, ultrasound-guided, durable central venous access in newborn piglets weighing 1 kg with a novel method of tunneling the catheter subcutaneously using a needle. Catheters (3–5 Fr, single- or double-lumen) were successfully placed in the right jugular vein of eight piglets weighing 1.3 ± 0.4 kg (mean ± standard deviation) with an average duration of catheter patency of 4.5 ± 2.1 days. There were no adverse events from catheter placement, though one catheter was inadvertently removed. This technique is a safe, minimally invasive method for obtaining secure central venous access in a neonatal porcine model.
10 May 2022, 12:27 am
Laboratory Animals
Laboratory Animals, Ahead of Print.
ObjectiveThe objective of this work was to anatomically locate the urethral orifice in female minipigs and describe the use of video laryngoscopes in urethral catheterization.MethodsUrethral catheterization guided by a video laryngoscope was attempted in 16 adult female Bama minipigs. The anatomical location of urethral orifices, operating time and complications (mucosal edema and bleeding in the vaginal vestibule, and the numbers of red blood cells (RBCs) and white blood cells (WBCs) in mid-stream urine samples) were recorded.ResultsThe anatomical location of the urethral orifice: the depth of the urethral orifice in female Bama minipigs was 4.2 ± 1.2 cm; all the urethral orifices were covered by mucosal folds of the vaginal vestibule. In the supine position, the orifice of the urethra at 9–12 and 1–3 o’clock accounted for 6.25%, 6.25%, 18.75%, 50%, 12.5%, 6.25% and 6.25%, respectively. All animals were successfully catheterized and the operating time was 9.0 (6.0–12.8) min. Complications: no bleeding in the vaginal vestibule was observed; the incidence of mucosal edema was 12.5%, all of which were mild; of urine samples collected 1 h after catheterization, 12.5% were found to contain RBCs and no RBCs were detected 6 h after catheterization; no WBCs were detected 1 h or 6 h after catheterization.ConclusionsThe urethral orifice of female minipigs was located deep in the vagina at variable clock directions and was unexceptionally covered by mucosal folds. Applying a video laryngoscope in urethral catheterization allowed quick and accurate exposure of the urethral orifice and minimal operational injury in female minipigs.
10 May 2022, 12:24 am
Laboratory Animals
Laboratory Animals, Ahead of Print.
6 May 2022, 12:13 am
Laboratory Animals
Laboratory Animals, Ahead of Print.
4 May 2022, 9:18 pm
Laboratory Animals
Laboratory Animals, Volume 56, Issue 2, Page 201-203, April 2022.
4 May 2022, 9:18 pm
Laboratory Animals
Laboratory Animals, Volume 56, Issue 2, Page 200-200, April 2022.
4 May 2022, 9:18 pm
Laboratory Animals
Laboratory Animals, Volume 56, Issue 2, Page 204-205, April 2022.
4 May 2022, 9:17 pm
Laboratory Animals
Laboratory Animals, Volume 56, Issue 2, Page 206-206, April 2022.
7 Apr 2022, 9:57 pm
Laboratory Animals
Laboratory Animals, Ahead of Print.